Composite

Part:BBa_K3262002:Design

Designed by: Shrihari Negi   Group: iGEM19_SNU_India   (2019-10-16)


J23115+Estrogen-SensitiveT7RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 819
    Illegal XhoI site found at 800
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2315
    Illegal AgeI site found at 1418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1145
    Illegal BsaI.rc site found at 2606
    Illegal SapI.rc site found at 560


Design Notes

Derived from information given on part page BBa_K1732015


Source

DNA synthesis

References